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How upgraded the organs are only affect how well you heal the soul, and by that giving your more souls in return.
Since your bottleneck will be how many souls you can have before reaching max, I say don't bother upgrading the organs until you unlocked the Cultist perk. You are not that much in need of the remote and automatic work in the early or mid game anyway. Stuff like cooking and smelting iron is free anyway.
Max Soul Gratitude is based off of the items in the Soul Room so I always try to build everything as quick as possible (although I do hold off on the Soul Containers until I can build the best ones because it's a hassle breaking them down and rebuilding them. I also skip level II on the first machine that removes souls and upgrade to III directly).
When wanting to use remote control with your map, remember that you need to build one in the Soul Room as well as any area you want access to or it won't work. It can be very helpful for things that take a lot of time to do, like pressing apples in the Vine Press or making paper at the Church Workbench. If you run out of Soul Gratitude, the task will pause and wait for you to get more.
So what you're saying is that I need bodyparts with a positive number of skulls, rather than a negative number? And that I don't need bodyparts with the exact number of skulls as the sin, just the same number as a minimum?
I unlocked everything in the Soul room a while ago, and have been healing souls for days now, so everything else isn't exactly new to me, and I've just been using random bodyparts in the machine. But I'd like to actually try and perfectly heal a soul for once, hence why I wanna know exactly how it works, so I know how to prepare my bodyparts for the machine.
On the other hand, I don't care too much about soul gratitude when it comes to remote control.
"Matchîng" more skulls in the healing doesn't provide more sin shards, just more soul gratitude.
The maximum sin shards is reached by having anything in the 7 slot (even organ with 0/0 skull will still provide their corresponding sin shard).
You can also leave the "healed soul" to decay in a chest for 1 extra sin shards instead of sending it into the portal for soul gratitude.