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Isshin is the leader of the Ashina clan, and has taken this land by force in his rebellion, which was shown in the intro cinematic. This is why the ministry is fighting him - they want the land to not be in the hands of some rebels but under their control. Genichiro is his adopted son, and has taken over command because Isshin is old and sick. Emma is Isshins doctor.
Genichiro has forsaken honor in favor of using any advantage he can, to win the war against the ministry. That is what he wants from Kuro. He does not want Kuro dead, but convince him to give Genichiro the "gift" of immortality, so he can not be defeated. Kuro is sort of a vassal of the Ashina clan, not an enemy.
Owl also wants immortality, but in contrast to Genichiro not to save his land and people but to take over everything. He instigated the attack on Hirata Estate, where Sekiro died the first time. Kuro saves him here by giving him immortality. Owl likely planned this, to later convice Sekiro through the Iron Code, to serve him instead of Kuro, and be used as a weapon.
No one has a problem with Sekiro directly, but you are sworn to serve Kuro. Genichiros men don't want you to save Kuro from Genichiro, so they fight you. Except for the few that talk to you, who may or may not know you form before the war, when you were serving a lord of the land. The ministry is not interested who you or Kuro are, you don't serve them, you die.
Isshin is too honorable to even consider doing what Genichiro does, and does not like it, So he sends his doctor to help you, without telling everyone that there might be disagreement in the clans leadership, for obvious reasons.