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Are all the wolves the same species?
Is the sex ratio ideal?
Was the wolf already injured from a previous fight or illness and then fought for alpha status?
Same thing with the tigers. Bengal and Siberian tigers are subspecies of tiger, and they don't go in the same habitat either.
Another real world reason could be suggested that the better zoos concerned with species preservation tend to not mix subspecies.
I think that's a decision that should be left up to the players. Yes, they're different subspecies, but unlike with the tigers (at least that I can think of) there are examples of arctic wolves and timber wolves sharing a habitat without issue. Mixbreeding isn't in the game anyway so that shouldn't matter much.