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1. Have enough polluted water.
2. Don't have high pressure of other gasses (specially CO2). This will set up right above the water and prevent it from giving polluted oxygen.
3. How many pufts can your polluted water/polluted oxygen sustain?
4. Is the polluted oxygen going somewhere else?
Theory... I might be able to make the next pen by going to a place infested with slimelung, P-water, and and a wild puft, and just plant a stable there and slowly block off the area with tiles to fit the stable maximum space count. Unsure if that area will get drained of P-water and P-oxygen, though. I had one successful farm that way, but I'd rather find a way to do it so my character(s) won't have to take a long trek to get to the pen and back to the base.
2. You could also put a a storage box in the room and fill it with polluted dirt to help off gas.
3. Why do you need slime?
Now that I'm thinking about it, I don't think I've had more than 2-3 pufts at a time either, but I do know that I've never pumped outside p-oxygen into their rooms. Typically just carve whatever p-water bowl existed wherever I found them, such that slime excretions would only ever fall into p-water below, never on a solid dry surface.
Build a reservoir, pump it full of p-water, then deconstruct it. You'll be left with all of that p-water in a bottle... which off-gasses much faster. Leave the pipes in place; if/when you need more p-oxygen, just rebuild the reservoir and do it again.
CO2 is heavier than O2 or p-O2, so just put a carbon skimmer and a water sieve in a loop at the lowest point in the area to handle it.
That is, run a pipe from the skimmer's output to the sieve's input, fill that with p-water, disconnect the bridge used to fill it, then run the pipe from the sieve's output to the skimmer's input. It will run forever from that point on, needing only power and sand.
If you really want to produce a ton of p-O2, I saw some guy in a YouTube video pumping it into a single layer of empty tiles, completely surrounded by airflow tiles. It can then offgas in every direction (which is why pools don't work so well; the liquid (or solid tiles) below and on either side eliminates 75% of the liquid's surface area). As it offgases, the pump pushes more into the space, so it's always at maximum pressure... which means it's constantly trying to make the air around it be at the same pressure as itself; ie, 100kg per tile.
He had a bunch of deodorizers turning it into "clean" O2, but there's no reason it wouldn't work just as well for keeping pufts fat and sassy.
I mean the offgassing in all directions thing. To me it seemed like p-water will only ever offgas to the top, even when completely surrounded by low pressure po2 (I have built things like this).
(The 'new-ish' (introduced with dlc) polluted dirt and polluted mud tiles will offgas in any direction)
Otherwise that build works nicely as offgassing polluted water is proportional to its' mass (haven't tried the infinite storage scenario, yet), but the gas pressure limit is still 1800g, so...
...this is just wrong.
Edit: you can have singular low pressure co2 (chlorine, anything heavier than po2) tiles roaming around the lakes surface to trick pressure checks. That's what happens in highly pressurized slime biomes.
Edit2: What I've never tested (thanks for sparking that idea), maybe p-water can offgas in all direcetions, but not a tile in multiple directions at once. So maybe I test with two layers of p-water (on airflow tiles).
Edit3: Though, my expected result would be the lower layer being completely inert. (Maybe I did test that at some point)
You're right, though, I shouldn't post before I've had my coffee... I explained that very badly.