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1) Spawn morbs - they produce polluted oxygen. You need a lot of them and you need to keep gas pressure low enough for them to keep it producing. Expect huge game performance drop when having so many critters.
2) Polluted oxygen vent - it can give you some gas to feed pufts
3) Having an open tank with pwater will emit polluted oxygen as well.
Puffs also only generate a small amount of slime per critter so you'll need a lot of them if you are using a lot of slime. My current puff farm can't even keep 1/10th of my mushrooms fertilize fully (4 puffs and a ton of morbs).
You want to keep a puft, puft prince, and dense puft together. They will produce more dense pufts that way and dense pufts are easy to keep.
A small pool of polluted water just below their pins also compost bins or outhouses in their pins can keep the pufts from starving. finding a polluted oxygen geyser can help a lot or sweeping slime and polluted dirt into their pins is also an option...