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Also remember some furniture/ workplaces (ie the butchers s table and the crematorium) give a dirtyness penalty for said room so watch out for that.
#1 Absolutely. A "sterile" room gets a bonus in medical outcomes and research.
#2 Depending on your room size and how clean you keep it, you may not need to cover the entire area to get to "sterile".
Other tiles (steal silver...) the same effect, but to a lesser amount.
Or if you are more of a numbers type you can look a this:
https://rimworldwiki.com/wiki/Room_stats#Cleanliness
Makes colonists clean up the work area before doing the job.