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You need every other weapon, passive and arcana to unlock the queen, including the Gemini weapons.
You need to unlock all the various weapons, Arcana, etc. EXCEPT the queen's weapon (which does have a slot in the collection, but doesn't count towards if it is 'complete' or not for unlocking the queen) in order to unlock the queen.
You then use the now unlocked queen to kill 100,000 enemies for that last unlock... and presumably whatever it unlocks doesn't appear in the collection.
or at least, such is my understanding.
1. Unlock the secret character Big Trouser and play with him at least once to add his starting weapon - the Candybox - to the list.
2. Unlock the "! - Gemini" arcana and combine it with the Gatti Amari weapon (you can just start any map with Giovanna, pick the card, then quit) to add Flock Destroyer to the list.