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You can confirm this by checking the Boon Info screen (Back button on gamepad). The bonus should no longer be there once you un-equip the Keepsake.
Unequipping is a good option if your Tartarus run didn't go that well and you didn't rack up much of a damage bonus, but once you've sufficiently charged up your Pierced Butterfly it's generally good to stick with it for the duration of your run.