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I am giving him a +rep because he perfected those back strokes and left me, where I couldn't walk for 5 days straight.
I am giving him a +rep because he perfected those back strokes and left me, where I couldn't walk for 5 days straight.
Schwartz/Mann. Hemoglobin and human serum albumin were obtained
from Nutritional Biochemicals Corporation. Protein solutions were prepared in 0.15 M NaCl. Concentrations were determined for bovine serum
albumin, human serum albumin, chymotrypsinogen A, and cytochrome c
spectrophotometrically in a Bausch and Lomb Spectronic 200 uv
spectrophotometer based on E~~,,+z = 6.6 (19,20), 5.3 (19,21), 20 (19,22)
and 17.1 (23,24) respectively. Hemoglobin solutions were prepared
gravimetrically.
Preparation ofprotein reagent. Coomassie Brilliant Blue G-250 (100 mg)
was dissolved in 50 ml 95% ethanol. To this solution 100 ml 85% (w/v)
phosphoric acid was added. The resulting solution was diluted to a final
volume of 1 liter. Final concentrations in the reagent were 0.01% (w/v)
Coomassie Brilliant Blue G-250, 4.7% (w/v) ethanol, and 8.5% (w/v)
phosphoric acid.
██]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]] 10% complete.....
████]]]]]]]]]]]]]]]]]]]]]]]]]]] 35% complete....
███████]]]]]]]]]]]]]]]] 60% complete....
███████████] 99% complete.....
🚫ERROR!🚫 💯True💯 Daddies are irreplaceable 💖I could never delete you Daddy!💖 Send this to ten other 👪Daddies👪 who give you 💦cummies💦 Or never get called ☁️squishy☁️ again❌❌😬😬❌❌ If you get 0 Back: no cummies for you 🚫🚫👿 3 back: you're squishy☁️💦 5 back: you're daddy's kitten😽👼💦 10+ back: Daddy😛😛💕💕💦👅👅
They don't know that
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