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2. Select frames 1-500 and import them
3. Create a second, new document
4. File>Import Video Frames to Layers....
5. Select frames 501-1000
6. select all the layers, Layer>Duplicate Layers... and duplicate the layers from from the second document into the first.
In photoshop, click on file>open and chose the first png created by your video software. Before importing the file make sure that "this is a sequence" (or something similar) is checked. This option is near the "ok" botton at the bottom-right corner of the window. When checked, photoshop import every picture in the folder and place it on a timeline. When this is done, if the timeline doesn't show up, go to window>timeline. This will open the timeline.
Now you can to save as web with any problem