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However, there will be a performance loss for a PCI Express 3.0 system, but it won't be from the lower bandwidth capability of PCI Express 3.0 itself, which the GPU will almost never need, but instead it will be from whatever CPU you have.
Since the lowest end RTX 50 series GPU out now is the RTX 5080, and the fastest PCI Express 3.0 CPUs are, what, the Intel 9th/10th generation CPUs (?), I'd say you're going to lose more performance from your CPU. Unless you're asking about the upcoming RTX 5070/5070 Ti, which may be a bit better on such CPUs.
At least for RTX5090 for now.
The oldest CPU I can see that would be OK would maybe be 12th Gen i7