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Probably, I can't find anything on ASUS' website but I have the same "issue" on my motherboard. Most motherboards, when a certain M.2 slot is populated, either a SATA port or PCI slot is disabled.
M.2_1 conflicts with nothing when running NVME, but takes out SATA posts if running in SATA-6G mode.
M.2_2 takes out sata 5/6 regardless of the flavor of m.2 just by using it.
Neither *should* be taking down your audio card...
That said, you are out of PCIe lanes... I would start jumping around on the x1 slots to see if one will work for you. No matter what you are likely going to have to deal with the GPU being bumped down to an x8 link if you want to give 4+4+1 away to other hardware.
For some reason Intel thinks consumers dont need more than a single x16 link on the CPU or about that. Now you have to shuffle the ones you have around a bit (if you have the ability to).
There is no way to change pcie slot one from x16 in the bios it is locked to that.
Having 2 m.2 and running the second pcie slot at x4 will stop pci slot 3 and 4 from working so basically if you have just one graphics card the only things that will get disabled are the sata slots although sata slot 1 and 2 will still work .
I just tried my soundcard in pci slot 4 and it shows up again thanks for the help everyone.
For future reference you may be able to set the M2 slot that disables Sata 5/6 to run in x2 mode. Least that is what happens on a TUF z270 Mark 1 board.
It's so owners have options. Not everyone will run Sli/crossfire but boards have that ability for those that do. Not everyone will use 4 DIMM slots, onboard graphics multiple USB 2, USB3 and USB 3.1 headers or onboard sound but boards have them anyway. Plus it's cheaper for manufacturers to produce thousands of board with them and give users the option to utilise the features how they want than it would be to make
Board 1 with 1 M.2 slots and 6 sata ports and 2 Dimm slots
Board 2 with 2 M.2 slots and 6 sata ports and 2 Dimm slots
Board 3 with 2 M.2 slots and 4 sata ports and 2 Dimm slots
Board 4 with 0 M.2 slots, 6 sata ports and 2 Dimm slots
Board 1 with 1 M.2 slots and 6 sata ports and 4 Dimm slots
Board 2 with 2 M.2 slots and 6 sata ports and 4 Dimm slots
Board 3 with 2 M.2 slots and 4 sata ports and 4 Dimm slots
Board 4 with 0 M.2 slots, 6 sata ports and 4 Dimm slots
and so on..........
Kinda like how they lock overclocking behind a K series cpu...
They like to draw lines in the sand that cost the consumer more money to cross.
1. It's not just Intel, it's AMD too. My X470 AORUS GAMING 7 WIFI has 2 M.2 slots, and using one of them disables the bottom-most PCI-e x16 (x4 bandwidth) slot. The chipset and CPU only have so much bandwidth, and in the chipset's case, chipsets with much more bandwidth need more power and more cooling.
2. It's also not up to Intel and AMD on which feature gets disabled to make bandwidth space in the chipset for the M.2 drive. It's up to the board manufacturer for that. ASUS usually only leaves that to SATA, which appears to be the case for the TUF PRO GAMING.
Neither the board vendors nor the user have to compromise with the CPU's have enough PCIe lanes native and the motherboard includes reasonable aditional lanes for its design,
The point I was making though was the generally factually correct point that Intel locks PCIe lanes behind pay walls where AMD CPU's across the board have more PCIe native to the user at all levels from entry to HEDT.
Perfect example is the 10700k @ 16 lanes vs the 3700x @ 24 lanes (and 4.0 to boot).
right out of the game AMD gives you 8 more lanes on the CPU. Thats more than enough to run an x16 native card plus 2 NVMe straight from the CPU, or 4,0 x8 plus 3 x4 nvme plus another 4 left over still to split to other devices.
Intel locks PCIe lanes behind a paywal, AMD doesnt, widely known fact just like Overclocking.
the onboard m.2 slots only disable the sata ports
it can use the pci-e x16 nvme card adapter in pci-e x16 style slots
https://www.amazon.com/ASUS-M-2-X16-V2-Threadripper/dp/B07NQBQB6Z
the last x16_3 slot only has x2 electrical, so the card adapter will only work with 2x nvme ssds
It turned out my SXR is faulty it shows up in device manager and loads the drivers but it wont play sound,i have just ordered an Soundblaster AE-7 it is the same price as a replacement SXR so i might as well get the newer one.