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Later you can use the "Big Daddy bomb" (place many proximity mines on a trash can and throw it with telekinesis)
Always hack all security in an area before you fight him.
I also used the electric trap tactic like Dufinstien.
Harvesting Little Sisters gives more ADAM, saving them gives less but for every three you save you get a reward containing some aditional ADAM and unique Plasmids and Tonics.
Harvesting 3 gives 480 ADAM, saving 3 gives 240 + 200 as reward + 2 other things as reward (often a Plasmid or a Tonic, rare ammo, EVE hypos, first aid kits)
Since you have dealt with them in both ways already you will get the neutral end regardless.
There are 21 in the game. so if you want the rewards save 17 and harvest 2 since you won't receive the last reward anyway.