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Tutorial (3 epidemics)
Introductory (4 epidemics)
Standard (5 epidemics)
Heroic (6 epidemics)
Legendary (7 epidemics)
The more players (up to 5), the harder.
Also adding the following game modes increase drastically the difficulty:
-Virulent Strain Epidemics
-Lab Board
-Hinterlands
-Emergency Events
-Mutation Challenge
-Mutation Challenge: Worldwide Panic
-Bio Terrorist
-etc
Only optional item that makes the game easier is the Quarantine Markers.