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Get yolo, the mutation that increases HP by 30% and then either a)2 grenades or b)2 of any other sub weapons and get the mutation to decrease cool-down for them. Focus on purple builds for the best overall damage. Double bow and quick bow are decent. Frost blast that causes bleeding and hakuto's bow are even better. A red build of infantry bow with +100% dmg from fire with a torch and 1 ice grande and 1 cluster bomb is devastating too.
Gauge how many hits each enemy takes based on your build once you get in a biome. If they die fast enough, bring up HP. Otherwise, damage goes up first. Once scrolls don't give more than 8%+ HP by the last stage either a)bring up attack more if you feel weak damage wise or b)skip them so that the HOTK and the enemies he spawns don't start doing MAJOR damage amounts by more than the HP you gain since everything scales when you change biomes (a bosses stage counts as a new biome).
BTW if you want TONS of cells without rushing use this route: prisoner's quarters, promenade of condemned, prison depths, ossuary, boss1, slumbering sanctuary, then pick whatever after. But this way before the 1st boss, you get an extra stage in. Plus the sanctuary has a cell canister you can grab for free. Save as much gold as you can to open golden doors in the prison depths, forgotten sepulcher, the castle, etc to get even more cells (8500 gold each). Yes, you can break the doors, but that curses you to have to kill 25-30 enemies to break the curse for the ones with cells behind them. Not worth it so late in the game, just pay instead.