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I dont know what you means with isolation chamber. It is the room number 5 in the jail? There is a security console, open it through the console.
The repair then hack is for the escape through the mimic portal. That I've done, I'm trying to do his special mission.
The isolation chamber is the larger area beyond the 5 locked rooms.
Still the devs are sadists with this title. I've replayed some portions too many times
volunteer can move under ground like Moon Shark typhon. Just use several neuromods to investigate it.
The energy sword will temporarily disable the locked gates & let you through.