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If it isn't opening in those conditions, then there's some mod conflict that is preventing it from opening, because I believe the only time in-game where it doesn't open once you have it unlocked is during challenge/daily runs.
It will always be open as long as you've beat the boss/entered the cleared boss room before 30 minutes. The only exception is if you manually open it with Mama Mega or the buffed Mega Bean in Repentance+.
I have always just done the boss rush, or started it and teleported out
I thought the 30 minute timer was for the It Lives! boss fight alone
not the entire run
Skimming through the wiki and online guides didn't specify that
It makes so much sense
Like I thought it was really weird that the conditions seemed way too easy and yet I still couldn't meet them