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Things that prevent achievements and progress:
I also usually add "antivirus blocking the game", because it happened for some people. Quite rare though.
If I had to guess, I'd say OP either uses seeds or mods (in the mods menu, AB+ only) too soon. Those mistakes are very common for new players.
Irrelevant, can't affect unlocks. ;-)
Edit:
Yay, I took too long to write! Woooo! :v
Usually, special seeds that make the game easier prevent unlocks, and the ones that make the game harder or are purely cosmetic do not.
According to the wiki, CONF ETT1 does prevent unlocks (not surprising consideing its effect).
https://bindingofisaacrebirth.wiki.gg/wiki/Seeds