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The unlock won't count. To be honest, it's kind of easier to unlock The Lost in Rebirth due to the fact that there's no RNG involved.. Well, there is, but there's a few broken seeds that let you get where you need to with the characters.
The only proper run is the Azazel run you gotta do to Sheol to get Satan to kill you. That's it.
Just pray to RNG for now, you'll find it eventually. Sorry for the whole "Pre AB" talk, just a poor person's perspective on the matter.
The best way to find trinkets is to search for Mom's Box (shop item), which spawns a random trinket every time you use it.
Another way is to look for a fortune teller. Those are often in super secret rooms and will sometimes appear in arcades. They also can be spawned by the Wheel of Fortune card.
i got mine with about 34 eden tokens.
Play as Eden and restart until you get mom's box (you have to have enough tokens, and yes you can unlock The lost with any craracter, not only Isaac).
Now all you have to do is to get the missing poster using the mom's box and kill yourself in the sacrifice room, i just did it this way yesterday.