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1. High Temps
2. Stool Sample
3. Afraid of Sounds
4. Blood Sample
The player can test every single animal, or they can save time by only testing sick animals. However many animals don't show signs so it can be hard to determine who is sick. If you have four animals in an enclosure and two are throwing up and have wounds whilst the rest do not then you can extrapolate two things:
1. There is one sick animal
2. The other is infected
Rotavirus is a sickness that is diagnosed through positive stool samples, NOT high temperature. You can identify this in game through the diagnosis tab at the vaccine monitor.
The Sick animal will need Rotacare as indicated in the vaccine monitor vaccine description, however the infected animal will need Rotavirid Toxica as indicated by TJs recipe list that you can see updated in your vaccine terminal once you get the recipes.
So unfortunately without samples it might be impossible. (You wouldnt be able to know Rotavirus without the stool sample)
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TLDR: Many people just test every single animal in the enclosure, however if the symptoms are evident (for example vomit, scars, odd movements), it allows the player to diagnose faster. (Its the difference between taking 6 penguin vials of blood and only needing 2 because they are the only ones showing symptoms)
I dont believe the physical symptoms are a good indicator to use as diagnosis, only a hint for who is sick/infected. It might be more of a pattern thing, certain symptoms are more prevalent for certain sicknesses so you might just have to guess for that one achievement.
If I am wrong however it would be neat to ask others who got that one achievement. I cant see it being anything other than luck however.
I hope this clarifies things.
It is very much possible to determine the disease an animal has without any official samples, it just takes a looong time, because you have to make a cheat-sheet. These are the symptoms that I've found you can determine without taking actual samples:
-Temperature: Don't let the bar fill up, only look at the animals quickly to see if they are orange/red or green.
-Scars
-Mucus: Use UV light
-Puking
-Wheezing: Making noise with every step, waving their head side to side (depends on animal species), sometimes stopping to cough, howl or make a different kind of obvious noise.
-Fighting with other animals
-Being scared: Running away from the player at all times
(-Redness of the eyes: I have only seen that one on the penguins 100%, with the other animals it can be hard to determine because of the lighting/their size. You also need a healthy animal to compare their eyes and if every animal is actually sick or pretending to be you can't compare the eye color) Some penguins just have very red eyes, no matter if healthy or sick.
Any one combination of these unofficial symptoms correlates to one specific disease. For example: Only wheezing without any of the other symptoms (unsure of the eyes though) shows that the animal suffers from Ulcerative colitis. High temperature, mucus and puking (unsure about eyes again) shows that the animal suffers from Gastroenteritis.
So it can be done, it is just a ton of work until someone puts in the effort to release a complete cheat-sheet. And yes, this is actually how it works. I had two cases of Salmonellosis and in both cases the animals showed the same kind of "unofficial" symptoms so it isn't random.
I see, well thank you for clarifying this to me. I've always sped through the diagnosis and have such a fast system that I dont even look close much anymore.
Thank you for your time