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I find Focus, ACP, Range & Slow Mine to be a good all round combination, I know a lot prefer the Violator to Focus but I guess that's preference. If playing multi, a mind control tower near the ACP works wonders but I wouldn't sacrifice the Focus tower to bring it in solo play.
2) That being said, in general the gatling, violator, and rocket towers are the worst. The scatter laser pretty much always trumps the gatling, and the orbital strike relay pretty much always trumps the violator and rocket towers, which you can see both from the numbers analysis in the wikis, or just from playtesting them yourselves.
3) Cannon vs focus really depends on whether you've activated the +HP and +regen feats. If you have neither feat on, cannon is better. If you have both feats on, focus is better. If you have just one of those feats on, it's a toss-up. Makeshift cannon is also an interesting alternative to the cannon and focus, regardless of which feats you have on, but it's a bit of a game - stunning a mob is great, but accidentally deactivating is not.
4) Lightning, ACP, and slow field have the potential to be great, but only if they're being boosted by a range spire. If you or a teammate aren't bringing a range spire, lightning, ACP, and slow field simply won't affect enough mobs to be worth it.
5) The anti-air tower is the first and final word against flying mobs. It's so cheap and effective, and its damage scales up so quickly with overcharging, that if you just put one of them in the middle of a map, even without a nearby range or amp spire, it will handle almost all of the flying mobs for you, freeing up the rest of your towers to concentrate on the ground mobs.
Towers with higher DPS than Gatling: Cannon, Lightning, ACP (if multiple enemies are hit), Violator, Rocket (if multiple enemies are hit), Scatter Laser, Focus, Makeshift Cannon (if multiple enemies are hit), Friendship Laser, Anti-Air, Drone (if multiple enemies are hit), Orbital Realy Strike (if multiple enemies are hit)
Towers with higher DPSPR than Gatling: ACP (if multiple enemies are hit), Rocket (if multiple enemies are hit), Focus, Makeshift Cannon (if multiple enemies are hit), Anti-Air, Drone (if multiple enemies are hit), Orbital Realy Strike (if multiple enemies are hit)
The Gatling tower definitely does not have the highest DPS nor the highest DPSPR in the game.
Source: http://sanctum.gamepedia.com/Towers and mathematics
Gatling is most effective dps per resource till armor shows up then its completely useless. So in survival when mobs get 10+ armor (-3000 per shot)gatlings shoot fast but low damage meaning each shot is mitigated. Also as enemys have more and more hp it means focus will have to target enemys longer ensuring each focus beam reaches maximum potential. As each tick of focus gets stronger up to 5x original damage.
That's only if you don't consider range and armor. Gatling does little to no damage against armored enemies, and its range is smaller than the scatter laser (especially at level 3), which means the gatling's DPS is actually 0 for significant periods of time, while the scatter is still hitting mobs. Numbers on paper aren't everything; playtest a stage twice, doing everything the same except using gatlings one run, and replacing them all with scatter lasers the second run. Scatter lasers invariably trump gatlings in reality.
for me one of the best is the Lightning
if you want to know the functioning of all damage and I invite you to visit the best wiki page sanctum 2
http://sanctum.gamepedia.com/Sanctum_Wiki