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You have different sources of doom which all have different amounts of damage they do. Also It's Endless 4. In Endless status effects are less effective at higher levels.
I was overly simple in my math because I already did observation based testing and noticed that doom was not killing anything on my build even in non endless and simply went to get a screenshot at this point because I very conveniently leveled up with a showcase on a boss. However, with further clarification your explanations are wrong.
Doesn't explain why the instant kill doesn't occur at any point. Also its endless 1 not 4 I have 4 curses on none of which reduce my damage. The overwhelming majority of doom stacks are coming from my strongest skill because it has the highest activation frequency and overlaps. Even if 100% of them were from my weaker skills which are only half as much damage (I'm assuming less at 150 for simpler math and rounding down in all contexts) Thats 715 stacks at 150 damage thats 107k damage.
107k damage there is no number of less effect that would play any significance on endless 1 without completely bricking dot/doom builds within a handful of waves. (and upon seeking clarification via the official discord this less effect does not apply to damaging negative effects only damage amplifying negative effects).
It does however take into account armor. This would not explain as to why an instant kill never occurred from doom and the boss would have to have an insane amount of armor to totally ignore doom (which they don't). Considering it was endless one and they have a base armor of 100 with a bonus 100 from endless the damage calc would be as follows: 107k /(0.5 + 200/200) which would be 71.3k doom damage ignoring all of my ailments that increase damage taken on the target or reduce armor. So the boss in an unrealistically bad scenario should have instantly died from doom after the next dot tick. Except it didn't it died from poison ticking several more times. The doom instant kill is non functional on non-innate doom without allowing the doom stacks to run out of duration (works perfectly fine in my testing on skills that innately apply doom).
I swear that it's piling Doom from other sources into a separate stack which gets evaluated later in the tick, rather than counting all sources of Doom at once.
Doom's damage also doesn't appear to be multiplied properly by "all damage" modifiers, either.
There are all sorts of weird behaviors in the DoT system that just don't make a lot of sense, frankly. Poison / Burn largely work as advertised, and I'm just about at the point where I take only what's statistically reliable, lol.
Also, I don't know for sure if all Doom charges really have the fifth level.
You're just having confirmation bias. Turn on damage numbers and look at the purple numbers, they appear for me having doom from non skill sources. It always executes, which is why doom is quite good as it will always execute enemies.