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These spores slowly grew into small plagas parasites hooking into their spinal cords to take more and more control.
Others had more mature and bigger parasites planted into them to aggressively take control, basically crushing any form of complex thought/personality.
In these instances, the host pretty cannot retain a semblance of former self anymore and depending on which method we are talking about, become useless for complex tasks.
What they need with Ashley is for her to be fully infected by a mature parasite while having a semblance of their regular self, so things seem normal and she can then proceed to slowly infect others.
For this, they would need a different approach from the former. Inject a special strain of plagas directly and have them mature fully slowly (unlike the spore versions which cant grow that big) over time, taking more and more control, until full control is established while also keeping a semblance of their self intact.
This process is still taking place while we play the game, which is why we can still remove the parasite and can save her (and Leon).
This is pretty much all taken from information across notes found in the game.