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It has an explanation in game. If you click on the Radiance button when initiating a new run you can see the specific effects of each level.
It is challenge / difficulty level, like Slay the Spire Ascension levels. Each level introduces new and incremental challenge features.
I don't think it's been implemented yet.
Edit: It probably won't be.
In answer to your original question (sorry, BTW, I meant to do that earlier), I managed to beat night 19, the first in Act IV, but then got creamed on night 20 (which was even worse). I was playing a blood-focused vampire from the second clan, the one with the wings. I had focused on finesse (33% dodge), life steal, and constitution for sake of mitigation, and had a set of skills that let me buff armor as needed and leverage a really high crit chance (25% at start of fight).
It was terribly effective until night 20 when they all had massive thorns, so I basically couldn't do any damage without ripping away my own armor that I needed to survive their high number of physical-damage hits. Not sure how to deal with that.
First. The basics. This isn't a rougelike. It is a casino rougelight. So there are ways to play this game wrong and ways to play this game right. If you want to play blood, and want to have a chance to win every time, you have to play the asian lady. SOMETIMES you can get away with the umbrella lady and the chick with her tatas out. But, both of them still perform infinitely better on ferocity.
Secondly. If you aren't running the Legendary flowergem trinket you will not win many games in radiant. Rose+Invulnerable gem+Flower gem=completely broken trinket. Due to item drop rates this is one of the easiest trinkets to get (2nd behind the useless double dagger trinket). Dispels debuffs and dots on your character (not silence for some reason), adds 3 block stacks and gives 5 thorns.
Finally, the fight. Kill the blue ones first. They attack every turn. the red ones only attack once every three turns. Save your defense for when it is going to be most useful. Blocks used for big damage but few attack chains, armor used for the opposite. Stagger them. Second. Blood basics pierce, ferocity basics cleave. Don't use them if they are going to hit the red duelist when they are riposting. UNLESS you can get enough physical armor stacks to cover the return damage AND have kill potential. And save your big damage for after the red ones attack, you'll deal double damage.
More general tips. Blood builds love crit. Stack blood early. There is a trinket to help you. Bats is an always pick ability, so is riposte. Asian lady's special always crits. Stack blood, use Asian ladies special, riposte until all enemies are dead.
Ferocity builds. Either focus lifesteal, thorns or finesse. Thorns is always the strongest option cus the punch ability that adds 15 damage per thorns stack, casts for 1 AP, and can be multicast for free up to 5 times is unhinged. Bone wheel and the basic stack armor can easily stack 100-200 thorns a turn. And there is another buff skill that looks like a demon porcupine that also gives 0 ap thorns stacks. Also, always run focus on ferocity. It is beyond broken.
And the final, most important tip. You do NOT have to spend your points every night. Every night that passes refreshes all point tabs. Allowing you a better chance to get what you want. ALSO! GOING TO THE SHOP ALSO REFRESHES SKILL, TRINKET AND TRAIT CHOICES! So if you don't see something you like, pop into the shop 1st and try again. This may be obvious to some people. But the game doesn't tell you, nor recommend it.