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You get the purple meter filled by opening purple crystal chests, activating the purple crystals, and a tiny bit for opening purple crystal doors and I think for clearing a room without getting hit.
If you shoot any of the purple crystals / doors / chests, they turn red. If you touch them, you take damage but open them without crystals and it fills your meter red. I think it also fills a bit as you take damage from enemies. You will lose this purple bar easily if you accidentally hit a red crystal object.
The game refers to this as the wisdom / violence bar. After you fill it up you can go to any teleporter and you should see a room disconnected from the rest of the map - an arrow usually points you to it.
If you filled wisdom (purple), you get a choice of 1 item from 2 different items. If you filled the violence (red) bar, you must permanently sacrifice a heart container, but get a choice of 1 item from 3 different items. It's generally easier/faster to get the red bar filled, but costs health (both in touching the red items as well as permanently from taking an item).
There really isn't though. Or what are you implying?
The fourth and fifth bosses are unlocked by taking two red deals or three purple deals respectively in one run (after beating Zeus for the first time) a token will spawn in the room after taking the last needed item that automatically add another level after beating Zeus again.